I have the oasis starter bacteria misappropriated something and in my 240l Aqarium, in the romp goldfish and Shubunkin, tried. For this, about four teaspoons of very fine substrate was loaded into a cloth bag and double those placed in front of the sponge of my outer filter. I have only one test for nitrite levels and tested these before and 10 days later, and this was actually dropped significantly, I had long ceased tested and I was not aware that the value was quite high. Thus, it works and I am very satisfied!